addgene provided pcdna3 mruby2 (Addgene inc)
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Addgene Provided Pcdna3 Mruby2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 80 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcdna3+mruby2/pcDNA3-mRuby2+(Plasmid+%2340260)/bio_rxiv__64898__2026__03__11__711183-172-0-0
Average 93 stars, based on 80 article reviews
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Plasmid Preparation:Article Title: Convergent activation of Ca 2+ permeability in two-pore channel 2 through distinct molecular routes Article Snippet: .. The coding sequences incorporating a 3' (Gly-Ser-Ala)3 flexible linker were subcloned into Article Title: Three-component CRISPR/Cas complex system and uses thereof Article Snippet: A fusion PCR of 5′ fragment of NLSPUFa with 3′ fragment of NLSPUb was used to create pAC1358:pmax-NLSPUFc_VP64. p65HSF1 activator ORF was amplified from MS2-P65-HSF1_GFP (Addgene: 61423) with FseI PacI sites to replace VP64 fragment in pAC164 to create pAC1410:pmax-dCas9_p65HSF1, and replace VP64 in pAC1355 and pAC1358 to create pAC1393: pmax-NLSPUFa_p65HSF1 and pAC1411:pmax-NLSPUFc_p65HSF1, respectively. .. Clover and mRuby2 were amplified from pcDNA3-Clover (Addgene #40259) and Article Title: Exosomes as Conduits: Facilitating Hepatitis B Virus-Independent Hepatitis D Virus Transmission and Propagation in Hepatocytes Article Snippet: .. The plasmids pCIHD24 and pCIHD27 were used for the expression of S-HDAg and L-HDAg, respectively, as previously described [ , ]. pCIneo and peGFP were purchased from Promega and Clontech, respectively. Article Title: Tissue‐like environments shape functional interactions of HIV ‐1 with immature dendritic cells Article Snippet: .. The plasmid pmRuby2.Vpr, encoding Vpr fused to mRuby2, was generated by excision of the eGFP coding region of peGFP.Vpr (McDonald et al , , a kind gift of Tom Hope) using AgeI and BsrGI restriction enzymes, followed by insertion of a PCR fragment encoding mRuby2 (Lam et al , ); amplified from Homologous Recombination:Article Title: Convergent activation of Ca 2+ permeability in two-pore channel 2 through distinct molecular routes Article Snippet: .. The coding sequences incorporating a 3' (Gly-Ser-Ala)3 flexible linker were subcloned into Amplification:Article Title: Three-component CRISPR/Cas complex system and uses thereof Article Snippet: A fusion PCR of 5′ fragment of NLSPUFa with 3′ fragment of NLSPUb was used to create pAC1358:pmax-NLSPUFc_VP64. p65HSF1 activator ORF was amplified from MS2-P65-HSF1_GFP (Addgene: 61423) with FseI PacI sites to replace VP64 fragment in pAC164 to create pAC1410:pmax-dCas9_p65HSF1, and replace VP64 in pAC1355 and pAC1358 to create pAC1393: pmax-NLSPUFa_p65HSF1 and pAC1411:pmax-NLSPUFc_p65HSF1, respectively. .. Clover and mRuby2 were amplified from pcDNA3-Clover (Addgene #40259) and Article Title: Tissue‐like environments shape functional interactions of HIV ‐1 with immature dendritic cells Article Snippet: .. The plasmid pmRuby2.Vpr, encoding Vpr fused to mRuby2, was generated by excision of the eGFP coding region of peGFP.Vpr (McDonald et al , , a kind gift of Tom Hope) using AgeI and BsrGI restriction enzymes, followed by insertion of a PCR fragment encoding mRuby2 (Lam et al , ); amplified from Cloning:Article Title: Three-component CRISPR/Cas complex system and uses thereof Article Snippet: A fusion PCR of 5′ fragment of NLSPUFa with 3′ fragment of NLSPUb was used to create pAC1358:pmax-NLSPUFc_VP64. p65HSF1 activator ORF was amplified from MS2-P65-HSF1_GFP (Addgene: 61423) with FseI PacI sites to replace VP64 fragment in pAC164 to create pAC1410:pmax-dCas9_p65HSF1, and replace VP64 in pAC1355 and pAC1358 to create pAC1393: pmax-NLSPUFa_p65HSF1 and pAC1411:pmax-NLSPUFc_p65HSF1, respectively. .. Clover and mRuby2 were amplified from pcDNA3-Clover (Addgene #40259) and Expressing:Article Title: Exosomes as Conduits: Facilitating Hepatitis B Virus-Independent Hepatitis D Virus Transmission and Propagation in Hepatocytes Article Snippet: .. The plasmids pCIHD24 and pCIHD27 were used for the expression of S-HDAg and L-HDAg, respectively, as previously described [ , ]. pCIneo and peGFP were purchased from Promega and Clontech, respectively. other:Article Title: Two-Photon Excitation Spectra of Various Fluorescent Proteins within a Broad Excitation Range Article Snippet: mRuby2 , Generated:Article Title: Tissue‐like environments shape functional interactions of HIV ‐1 with immature dendritic cells Article Snippet: .. The plasmid pmRuby2.Vpr, encoding Vpr fused to mRuby2, was generated by excision of the eGFP coding region of peGFP.Vpr (McDonald et al , , a kind gift of Tom Hope) using AgeI and BsrGI restriction enzymes, followed by insertion of a PCR fragment encoding mRuby2 (Lam et al , ); amplified from Polymerase Chain Reaction:Article Title: Tissue‐like environments shape functional interactions of HIV ‐1 with immature dendritic cells Article Snippet: .. The plasmid pmRuby2.Vpr, encoding Vpr fused to mRuby2, was generated by excision of the eGFP coding region of peGFP.Vpr (McDonald et al , , a kind gift of Tom Hope) using AgeI and BsrGI restriction enzymes, followed by insertion of a PCR fragment encoding mRuby2 (Lam et al , ); amplified from |
